Chloroquine embryotoxicity in the postimplantation rat conceptus in vitro

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Chloroquine embryotoxicity in the postimplantation rat conceptus in vitro.

The embryotoxicity of the antimalarial drug chloroquine (CQ) was evaluated in vitro using the rat whole embryo culture system. CQ was found to be embryotoxic and dysmorphogenic when added directly to the culture media containing gestational day (GD) 10 rat conceptuses. Twenty-six-hr exposure to CQ elicited dose-related decreases in embryonic crown-rump length, protein and DNA contents and incre...

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Chloroquine accumulation and alterations of proteolysis and pinocytosis in the rat conceptus in vitro.

The teratogenicity of chloroquine (CQ) has been hypothesized to result from its effects on lysosomal function, specifically the ability of the visceral yolk sac (VYS) to capture and degrade external macromolecules. Using the rat whole embryo culture system, we evaluated the ability of CQ to accumulate in conceptual tissues and its effects on aspects of VYS function known to be important in the ...

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the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation

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Development of the rat conceptus in vitro and associated changed in components of culture medium.

Rat conceptuses of pregnancy day 11 (embryonic age approximately 10-5 days) with embryos within the yolk and amniotic sacs and polar allantoic placental tissues were cultured by the 'New system' utilizing homologous serum in 60 ml roller bottles with 20% O2, 5% CO2 and 75% N2 gas environment (five conceptuses/10 ml serum). Embryonic growth and development was assessed by external morphology and...

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ژورنال

عنوان ژورنال: Teratology

سال: 1993

ISSN: 0040-3709,1096-9926

DOI: 10.1002/tera.1420480305