Chimeric 6‐methylsalicylic acid synthase with domains of acyl carrier protein and methyltransferase from Pseudallescheria boydii shows novel biosynthetic activity
نویسندگان
چکیده
منابع مشابه
Plant holo-(acyl carrier protein) synthase.
1. An improved method was developed for the assay of plant holo-(acyl carrier protein) synthase activity, using Escherichia coli acyl-(acyl carrier protein) synthetase as a coupling enzyme. 2. Holo-(acyl carrier protein) synthase was partially purified from spinach (Spinacia oleracea) leaves by a combination of (NH4)2SO4 fractionation and anion-exchange and gel-permeation chromatography. 3. The...
متن کاملPlant holo - ( acyl carrier protein ) synthase Salah
1. An improved method was developed for the assay of plant holo-(acyl carrier protein) synthase activity, using Escherichia coli acyl-(acyl carrier protein) synthetase as a coupling enzyme. 2. Holo-(acyl carrier protein) synthase was partially purified from spinach (Spinacia oleracea) leaves by a combination of (NH4)2SO4 fractionation and anion-exchange and gel-permeation chromatography. 3. The...
متن کاملMeningitis caused by Pseudallescheria boydii.
We present a 43-year-old immunocompetent man who developed meningitis caused by Pseudallescheria boydii. The patient had no history of near drowning, trauma, steroid administration, operations or any other underlying systemic disease. He presented with intermittent fever associated with headache, bilateral eye pain, and vomiting. Progressive hydrocephalus was noted during the course of the dise...
متن کاملMolecular recognition between ketosynthase and acyl carrier protein domains of the 6-deoxyerythronolide B synthase.
Every polyketide synthase module has an acyl carrier protein (ACP) and a ketosynthase (KS) domain that collaborate to catalyze chain elongation. The same ACP then engages the KS domain of the next module to facilitate chain transfer. Understanding the mechanism for this orderly progress of the growing polyketide chain represents a fundamental challenge in assembly line enzymology. Using both ex...
متن کاملCharacterization of recombinant thioesterase and acyl carrier protein domains of chicken fatty acid synthase expressed in Escherichia coli.
Fatty acid synthase of animal tissue is a multifunctional enzyme comprised of two identical subunits, each containing seven partial activities and a site for the prosthetic group, 4'-phosphopantetheine (acyl carrier protein). We have recently isolated cDNA clones of chicken fatty acid synthase coding for the dehydratase, enoyl reductase, beta-ketoacyl reductase, acyl carrier protein, and thioes...
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ژورنال
عنوان ژورنال: Microbial Biotechnology
سال: 2019
ISSN: 1751-7915,1751-7915
DOI: 10.1111/1751-7915.13445