Chikusetsu saponin IVA induces apoptosis and mitochondrial dysfunction of benign prostatic hyperplasia epithelial cell line (BPH-1) by inhibiting JAK/STAT3 signaling pathway

Purpose: To determine the potential effect of Chikusetsu saponin IVA (CS-IVA) on benign prostatic hyperplasia (BPH), as well mechanism action.
 Methods: Benign epithelial cell response to treatment by CS-IVA was evaluated 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation assay, and cycle analysis. Flow cytometry immunoblot assay were used assess apoptosis. Mitochondrial damage examined JC-1 staining immunoblot, while also performed JAK/STAT3 pathway.
 Results: inhibited growth progression BPH-1 but promoted apoptosis (p < 0.01). It exacerbated mitochondrial in lines With regard action, pathway cells 0.01).
 Conclusion: induces dysfunction inhibiting pathway, thus it is a drug for BPH. However, vivo studies are required validate these results