Chemiluminescent VEGF ELISA Using the SpectraMax® L Microplate Luminometer
نویسندگان
چکیده
منابع مشابه
Chemiluminescent ELISA for the BTEX determination in water and soil.
An indirect competitive chemiluminescence enzyme-linked immunosorbent assay (CL-ELISA) for the determination of benzene, toluene, ethylbenzene, and ortho-, meta-, para-xylenes (BTEX) in soil and water was developed. The assay was optimized concerning the coating conjugate concentration, anti-BTEX antiserum dilution, incubation time effect on primary and secondary antibody incubation, and temper...
متن کاملDevelopment of a chemiluminescence competitive PCR for the detection and quantification of parvovirus B19 DNA using a microplate luminometer.
BACKGROUND Quantitative PCR of viral nucleic acids can be useful clinically in diagnosis, risk assessment, and monitoring of antiviral therapy. We wished to develop a chemiluminescence competitive PCR (cPCR) for parvovirus B19. METHODS Parvovirus DNA target sequences and competitor sequences were coamplified and directly labeled. Amplified products were then separately hybridized by specific ...
متن کاملSpecific Detection of Full-length Glucagon From Blood Samples Using a Chemiluminescent ELISA Kit
Glucagon is the most important counter-regulatory hormone to insulin in blood glucose homeostasis. It elevates blood glucose levels by stimulating gluconeogenesis and glycogenolysis while inhibiting glycogenesis and glycolysis mainly in the liver1. Recently, glucagon and its receptors have been actively pursued as therapeutic target for diabetes, especially Type I diabetes2. Therefore, it is cr...
متن کاملAdaptation of the troponin T ELISA test to a microplate immunoassay reader.
Troponin T concentration in serum is usually measured by the automated method developed by Boehringer Mannheim for the ES-series of analysers. These instruments need at least 140 microliters of serum and 700 microliters of reagents for a single analysis, which takes 90 min. We describe an alternative procedure, using streptavidin-coated microtitre plates, troponin T reagents of Boehringer Mannh...
متن کاملFast chemiluminescent measurement of RNA polymerase activity based on photon counting technology.
A fast and simple assay for T7 RNA polymerase based upon chemiluminescent detection of the synthesized, digoxigenin-labeled RNA on a nylon membrane with anti-digoxigenin coupled alkaline phosphatase and CSPD as substrate is described. Activity of RNA polymerase is determined with high sensitivity by quantifying the emitted light of the microplate-formatted dot-blot membrane with a photon counti...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2008
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113044