Chemiluminescence detection of gangliosides by thin-layer chromatography.
نویسندگان
چکیده
منابع مشابه
Chemiluminescence detection of gangliosides by thin-layer chromatography.
We describe a method for the detection of gangliosides on thin-layer chromatography plates using enhanced chemiluminescence. In contrast to previously published colorimetric techniques, the use of chemiluminescence of detect antibody binding to gangliosides has increased sensitivity and simplicity. In addition, the use of chemiluminescence lacks the hazards of radioactivity, and produces a perm...
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A method for the separation and quantification of a complex ganglioside mixture from a clinically available amount (5 ml) of human cerebrospinal fluid (CSF) is described. After reduction of the CSF volume by ultrafiltration, gangliosides are extracted with methanol/chloroform, then separated and quantified by high performance thin layer chromatography (HPTLC) and direct densitometry. For purifi...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1995
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)40073-2