Chemiluminescence-Based Method for Genotyping Tg.AC Responder Mice

نویسندگان
چکیده

منابع مشابه

Chemiluminescence-based method for genotyping Tg.AC responder mice.

The Tg.AC transgenic mouse model (2) is under evaluation by the National Toxicology Program (NTP) as an adjunct to the conventional two-year bioassay for the identification of chemical carcinogens. The short-term, 26week bioassay relies on the empirical observation that Tg.AC transgenic mice produce skin papillomas when topically treated with chemical carcinogens (1,3). Ongoing studies in our l...

متن کامل

A Novel and Simple Method for HCV Genotyping

Hepatitis C virus (HCV) is a major global health problem and one of the most common causes of chronic liver diseases. The current study was carried out to investigate the ability of High Resolution Melting (HRM) method in HCV genotyping. 40 HCV-Positive sera with unknown and 12 sera with known HCV genotypes were collected from different clinical laboratories in Tehran as well as Rasht in north ...

متن کامل

SNP genotyping for the genetic monitoring of laboratory mice by using a microarray-based method with dualcolour fluorescence hybridisation.

Ensuring the genetic homogeneity of the mice used in laboratory experiments contributes to the Reduction aspect of the Three Rs, by maximising the quality of the data obtained from any animals that are used for these purposes, and ultimately reducing the numbers of animals used. Single nucleotide polymorphism (SNP) genotyping is especially suitable for use in the analysis of the genetic purity ...

متن کامل

A simple PCR-based method for the rapid genotyping of inherited fifth complement component (C5)-deficient mice

The fifth component of complement (C5) is considered to be the center of complement activation and function. However, there are no genetically engineered knockout mice for this gene, and the only commercially available inherited C5-deficient mice, in which a "TA" nucleotide deletion in the coding frame was previously identified, are in theC57BL/10Sn genetic background rather than the commonly u...

متن کامل

Terbium Sensitized Chemiluminescence Method for the Determination of Rabeprazole -Application to Pharmaceutical Analysis and Dissolution Studies

A simple and sensitive chemiluminescence-based method was established for the determination of rabeprazole.The proposed method was based on the enhancing effect of rabeprazole on Ce(IV)-Na2SO3 -Tb(III) chemiluminescence reaction. A possible mechanism was discussed for chemiluminescence system by studying UV-Vis, fluorescence and chemiluminescence spectra. The effects of va...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: BioTechniques

سال: 1999

ISSN: 0736-6205,1940-9818

DOI: 10.2144/99272bm13