Chemical modifications of artificial restriction DNA cutter (ARCUT) to promote itsin vivoandin vitroapplications
نویسندگان
چکیده
منابع مشابه
Homologous recombination in human cells using artificial restriction DNA cutter.
The double strand break induced by an artificial restriction DNA cutter (ARCUT) was successfully repaired in human cells with high frequencies through homologous recombination.
متن کاملChemical modification of Ce(IV)/EDTA-based artificial restriction DNA cutter for versatile manipulation of double-stranded DNA
A monophosphate group was attached to the terminus of pseudo-complementary peptide nucleic acid (pcPNA), and two of thus modified pcPNAs were combined with Ce(IV)/EDTA for site-selective hydrolysis of double-stranded DNA. The site-selective DNA scission was notably accelerated by this chemical modification of pcPNAs. These second-generation artificial restriction DNA cutters (ARCUTs) differenti...
متن کاملChemical and biological approaches to improve the efficiency of homologous recombination in human cells mediated by artificial restriction DNA cutter
A chemistry-based artificial restriction DNA cutter (ARCUT) was recently prepared from Ce(IV)/EDTA complex and a pair of pseudo-complementary peptide nucleic acids. This cutter has freely tunable scission-site and site specificity. In this article, homologous recombination (HR) in human cells was promoted by cutting a substrate DNA with ARCUT, and the efficiency of this bioprocess was optimized...
متن کاملCut-and-Paste of DNA Using an Artificial Restriction DNA Cutter
DNA manipulations using a completely chemistry-based DNA cutter (ARCUT) have been reviewed. This cutter, recently developed by the authors, is composed of Ce(IV)/EDTA complex and two strands of pseudo-complementary peptide nucleic acid. The site-selective scission proceeds via hydrolysis of targeted phosphodiester linkages, so that the resultant scission fragments can be easily ligated with ot...
متن کاملSite-selective scission of human genome by artificial restriction DNA cutter.
By using an artificial restriction DNA cutter which is composed of Ce(iv)/EDTA and two pseudo-complementary peptide nucleic acid strands (pcPNAs), only one target site in the whole genome of human beings (one site in the X chromosome) was selectively hydrolyzed.
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ژورنال
عنوان ژورنال: Artificial DNA: PNA & XNA
سال: 2014
ISSN: 1949-095X,1949-0968
DOI: 10.1080/1949095x.2015.1112457