Chemical Composition and Cytoprotective Activities of Methanolic Extract of Asplenium adiantum-nigrum L. (Aspleniaceae)

Ferns can be a source of polyphenolic compounds, with the fronds being main parts described in ethnopharmacological studies. The present study screened phytochemicals and evaluated vitro activities methanolic extract Asplenium adiantum-nigrum L. (AAM), an Aspleniaceae fern collected from Prades mountains (Tarragona, Spain). Phytocharacterization by HPLC-MS/MS confirmed that major flavonoids isolated AAM are flavanols while phenol acids, chlorogenic acid most representative one. Cytotoxicity, cytoprotection, cellular repair activity, phototoxicity were determined presence 0.01, 0.1, 1 mg/mL extract. No cytotoxicity was obtained any cell lines tested: non-tumoral (3T3 HaCaT) tumoral (HeLa, HepG2, A549) cells. Additionally, showed greater protective effect against H2O2 3T3 than HaCaT Despite low total phenolic content (1405.68 mg phytochemicals/kg dry extract), cytoprotective activity this could associated synergistic antioxidant action their profile. In addition, did not non-cytotoxic 1.8 J/cm2 dose UVA light both lines.