Characterization of the N-linked glycans of Giardia intestinalis
نویسندگان
چکیده
منابع مشابه
Characterization of the N-linked glycans of Giardia intestinalis.
This article reports the first rigorous evidence for the existence of N-glycans in Giardia intestinalis, a parasite that is a widespread human pathogen, being a major cause of enteric disease in the world. Excreted/secreted molecules of G. intestinalis are known to stimulate the immune system. Structural strategies based on MALDI and electrospray mass spectrometry were employed to examine the e...
متن کاملIntracellular functions of N-linked glycans.
N-linked oligosaccharides arise when blocks of 14 sugars are added cotranslationally to newly synthesized polypeptides in the endoplasmic reticulum (ER). These glycans are then subjected to extensive modification as the glycoproteins mature and move through the ER via the Golgi complex to their final destinations inside and outside the cell. In the ER and in the early secretory pathway, where t...
متن کاملThe Possible Pathogenicity of Giardia Intestinalis
Assistant Surgeon, Medical College Hospital, Calcutta, ortnerly Clinical Pathologist, Protozoology Depart-' ment, Calcutta School of Tropical Medicine.) The; role which Giardia inrcsnnuus may 'n the production of intestinal symptoms is still a disputed point. Nothing very definite can be stated one way or the other. It is claimed on the one hand' that it is frequently present in healthy persons...
متن کاملThe membrane potential of Giardia intestinalis.
Giardia intestinalis is a primitive microaerophilic protozoan parasite which colonises the upper intestine of humans. Despite the evolutionary and medical significance of this organism, its physiology is very poorly understood. In this study we have used a novel flow cytometric technique to make quantitative measurements of the electrical potential across the plasma membrane of G. intestinalis ...
متن کاملChanges in the N-glycome, glycoproteins with Asn-linked glycans, of Giardia lamblia with differentiation from trophozoites to cysts.
Giardia lamblia is present in the intestinal lumen as a binucleate, flagellated trophozoite or a quadranucleate, immotile cyst. Here we used the plant lectin wheat germ agglutinin (WGA), which binds to the disaccharide di-N-acetyl-chitobiose (GlcNAc(2)), which is the truncated Asn-linked glycan (N-glycan) of Giardia, to affinity purify the N-glycomes (glycoproteins with N-glycans) of trophozoit...
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ژورنال
عنوان ژورنال: Glycobiology
سال: 2004
ISSN: 1460-2423,0959-6658
DOI: 10.1093/glycob/cwi035