Characterization of the Bacillus subtilis tryptophan promoter region.

Characterization of the Bacillus subtilis tryptophan promoter region.

The nucleotide sequence of the control region of the trp operon of Bacillus subtilis has been determined. The region was shown to contain the trp promoter by deletion analysis and by determination of the transcription start site. The trp promoter shows similarity to the consensus sequence for Escherichia coli and B. subtilis promoters. The presence of the trp control region on a high-copy-numbe...

Promoter Activation in Bacillus subtilis

The isolation and genetic characterization of mutants of the tryptophan system of Bacillus subtilis.

I T has previously been shown in this laboratory (CARLTON 1967) and elsewhere ( ANAGNOSTOPOULOS and CRAWFORD 1961 , 1967) that mutations leading to defects in tryptophan biosynthesis in Bacillus subtilis map in a cluster on the genome of this organism, and in an order which corresponds to that obtained for similar mutants in Escherichia coli (YANOFSKY and LENNOX 1959) and Salmonella typhimurium...

Identification and nucleotide sequence of the promoter region of the Bacillus subtilis gluconate operon.

The nucleotide sequence (742 bp) of the promoter region of the Bacillus subtilis gluconate (gnt) operon is presented. Nuclease Sl mapping revealed the start point of the transcription and suggested that the expression of this operon is probably regulated at the transcriptional level. The sequences of the -35 and -10 regions suggested that RNA polymerase possessing sigma-43 may recognize this st...

Characterization of mutants with single and multiple defects in the tryptophan biosynthetic pathway in Bacillus subtilis.

Sixty-five tryptophan auxotrophs which map in a cluster on the genome of Bacillus subtilis were characterized on the basis of (i) growth response, (ii) accumulation of intermediate compounds, and (iii) determination of enzymatic defects. They could be placed into six phenotypic classes. Certain of the mutants exhibited pleiotropic effects on more than one enzymatic activity in a manner differen...