Characterization of α-rhamnosidase activity from a Patagonian Pichia guilliermondii wine strain
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چکیده
منابع مشابه
Upscale of recombinant α-L-rhamnosidase production by Pichia pastoris MutS strain
Pichia pastoris is currently one of the most preferred microorganisms for recombinant enzyme production due to its efficient expression system. The advantages include the production of high amounts of recombinant proteins containing the appropriate posttranslational modifications and easy cultivation conditions. α-L-Rhamnosidase is a biotechnologically important enzyme in food and pharmaceutica...
متن کاملMolecular typing of the yeast species Dekkera bruxellensis and Pichia guilliermondii recovered from wine related sources.
A total of 63 strains of Dekkera bruxellensis and 32 strains of Pichia guilliermondii isolated from wine related environments were identified by restriction analysis of the 5.8S-ITS region of the rDNA. These strains were subjected to intraspecific discrimination using mtDNA restriction and RAPD-PCR analysis. The isolates identified as D. bruxellensis yielded 3 different molecular patterns of mt...
متن کاملSpoilage yeasts in Patagonian winemaking: molecular and physiological features of Pichia guilliermondii indigenous isolates.
Yeasts belonging to the genus Dekkera/Brettanomyces, especially the species Dekkera bruxellensis, have long been associated with the production of volatile phenols responsible for off-flavour in wines. According to recent reports, the species Pichia guilliermondii could also produce these compounds at the initial stages of fermentation. Based on the abundance of P. guilliermondii in Patagonian ...
متن کاملValidation of the species Pichia guilliermondii.
Many species of yeasts exist in nature in haploid form. If strongly heterothallic, strains of such species produce no ascospores when tested individually. They do mate if sexually reactive strains of opposite sex are mixed on a sporulation medium. In 1952, L. J. Wickerham and K. A. Burton (J. Bacteriol. 63:449, 1952) reported that all strains of an imperfect form known variously as Dematium cho...
متن کاملCharacterization of an a-Amylase with Broad Temperature Activity from an Acid-Neutralizing Bacillus cereus Strain
Bacillus sp. GUF8, isolated from acidic soil samples of a tea farm was identified as Bacillus cereus, based on 16S rDNA sequencing and standard bacterial identification methods. Following optimization of enzyme production, the resulting α-amylase was purified by acetone precipitation and ion exchange chromatography. Consequently, thermostability and kinetic parameters of the purified enzyme wer...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2010
ISSN: 1364-5072
DOI: 10.1111/j.1365-2672.2010.04854.x