Characterization of recombinant MSP5 Anaplasma marginale Havana isolate
نویسندگان
چکیده
منابع مشابه
Characterization of recombinant MSP5 Anaplasma marginale Havana isolate
Anaplasma marginale is the causative agent of bovine anaplasmosis, a disease of worldwide economic importance. Major surface proteins (MSPs) are involved in host-pathogen and tick-pathogen interactions and they have been used as markers for the genetic characterization of A. marginale strains and phylogenetic studies. The major surface protein 5 (MSP5) is highly conserved in the genus Anaplasma...
متن کاملDevelopment and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardizat...
متن کاملAntigenic characterization of Brazilian isolates of Anaplasma marginale.
Antigenic characterization of Anaplasma marginale isolates, by identifying conserved and variable epitopes of major surface proteins (MSP), is an important tool for vaccine development against this rickettsia. The B cell epitopes of A. marginale isolates from three microregions of the State of Pernambuco and one from the State of Mato Grosso do Sul, Brazil, were characterized by indirect fluore...
متن کاملCharacterization of Anaplasma phagocytophilum major surface protein 5 and the extent of its cross-reactivity with A. marginale.
Major surface protein 5 (Msp5) of Anaplasma marginale is highly conserved in the genus Anaplasma and the antigen used in a commercially available competitive enzyme-linked immunosorbent assay (cELISA) for serologic identification of cattle with anaplasmosis. This study analyzes the degrees of conservation of Msp5 among various isolates of Anaplasma phagocytophilum and the extent of serologic cr...
متن کاملCatalase Activity in Anaplasma Marginale.
Wallace, W. R. (Louisiana State University, Baton Rouge), and G. T. Dimopoullos. Catalase activity in Anaplasma marginale. J. Bacteriol. 91:309-311. 1965.-Extracts of erythrocytes infected with Anaplasma marginale were found to contain more catalase activity than normal erythrocytic preparations. The increase in catalase activity appeared concurrently with increases in the number of erythrocyte...
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ژورنال
عنوان ژورنال: Brazilian Journal of Microbiology
سال: 2009
ISSN: 1517-8382
DOI: 10.1590/s1517-83822009000400032