Characterization of R-phycocyanin. Chromophore content of R-phycocyanin and C-phycoerythrin.
نویسندگان
چکیده
منابع مشابه
Characterization of R-phycocyanin. Chromophore content of R-phycocyanin and C-phycoerythrin.
R-phycocyanin was purified from two independent isolates of the unicellular red alga Porphyridium cruentum. At pH 7.0 the protein sediments as a single component with s 20,w of 5.98 S (at 2 mg/ml, gamma/2=0.02). Over a protein concentration range of 0.2 to 0.5 mg/ml (gamma/2=0.16), sedimentation equilibrium gave a molecular weight of 103,000 +/- 6,000 with no evidence of heterogeneity. In commo...
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The biliprotein allophycocyanin was purified from Phormidium luridum, Anabaena variabilis and Plectonema boryanum. R-phycocyanin was purified from Rhodymenia palmata. The chromophores were cleaved from the denatured protein by methanol hydrolysis. They were purified and crystallized as the dimethyl esters. Chromatographic and absorption-spectral (visible-ultraviolet and infrared) comparisons wi...
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A comparison between R-phycocyanin (R-PC)-labeled monoclonal antibody (MAb) probe and R-phycoerythrin (R-PE)-labeled MAb probe for the detection of the three standard reference strains of the cultured-derived Entamoeba histolytica trophozoites, namely HK-9, HM-1:IMSS, and HTH-56:MUTM were evaluated by using direct immunofluorescence antibody (DIFA) assay five times for each strain. Under the bl...
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The bilin chromophores of the a or /3 subunit of C-phycocyanin (PC) from Mastigocladus laminosus were modified, and subsequently recombined with the respective complementary unmodified chromophores. The modifications consisted of photobleaching (350 nm) or reversible reduction of the verdinto rubin-type chromophore(s). Recombination led to heterodimers (a& but the heterohexameric aggregation st...
متن کاملThree-dimensional structures of C-phycocyanin and B-phycoerythrin at 5-A resolution.
Single crystal x-ray diffraction studies are proceeding on two phycobiliproteins: C-Phycocyanin from Anabaena variabilis and B-Phycoerythrin from Porphyridium cruentum. C-Phycocyanin consists of six alpha and six beta subunits. A three-dimensional x-ray diffraction study of this light-harvesting protein (P6(3); a = b = 154.1 A, c = 40.1 A) at 5-A resolution shows that the molecule is 110 A in d...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1975
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)41208-8