Characterization of Pseudomonas syringae pv. morsprunorum originating from sweet cherry and plum in Serbia

Pseudomonas strains originating from symptomatic (bacterial spot) leaf tissues of sweet cherry (Topola, Sumadija) and plum (Krusedol Selo, Srem) were isolated during 2016 2020, respectively. Based on the findings yielded by classical microbiological methods, LOPAT (+---+), GATTa (--++) pathogenicity tests performed detached fruitlets (sweet sour cherry) pods (bean pods), all confirmed to belong P. syringae pv. morsprunorum. The detection cfl gene allowed that race 1 be identified. DNA fingerprinting patterns obtained with four rep-PCR (BOX ERIC), RAPD-PCR (M13), IS50-PCR (IS50) methods revealed seven tested s. morsprunorum strains, as well comparative KBNS71 reference strain CFBP 2119, genetically heterogeneous. Conversely, MLSA based four-gene-based scheme (gapA, gltA, gyrB, rpoD) indicated genetic homogeneity among Serbian NCBI. Although indicate used in this study are 100% identical, they might have different virulence genes, genome sequencing should eventually find sub-clades host.