Characterization of polysaccharide antigens of Streptococcus mutans B13 grown under various conditions

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Purification and characterization of a rhamnose-containing cell wall antigen of Streptococcus mutans B13 (serotype d).

A rhamnose-containing polysaccharide (RCP) was extracted and purified from cell walls of Streptococcus mutans B13 (serotype d) and was chemically and immunologically characterized. Walls were initially extracted with 5% trichloroacetic acid at 4 degrees C to remove the serotype antigen and were then sequentially extracted with increasing concentrations of hot acid. Extracts lacking galactose we...

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Enhanced toxicity of copper for Streptococcus mutans under anaerobic conditions.

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Serological purification of polysaccharide antigens from Streptococcus mutans serotypes a and d: characterization of multiple antigenic determinants.

The polysaccharide antigen preparations from serotype a and serotype d strains of Streptococcus mutans contained both a serotype-specific antigenic determinant and a common a-d antigenic determinant, as demonstrated by agar gel diffusion studies and a quantitative cross-precipitin assay. The chromatographically purified antigens were isolated by a method which depended on their serological spec...

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Wall-associated protein antigens of Streptococcus mutans.

When heat-killed whole organisms of Streptococcus mutans strain Ingbritt (serotype c) were injected into rabbits, antibodies to at least 12 antigens were detectable by crossed immunoelectrophoresis. In contrast, when rabbits were immunized with organisms which had been subjected to extraction with the detergent sodium dodecyl sulphate (SDS), antibodies to only two protein antigens were found. T...

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[Isolation and characterization of Streptococcus mutans].

s), vol. 2. Information Retrieval. Inc.. Washington, D.C. 23. Pollock, J. J., L. I. Katona, H. Goodman, M. I. Cho, and V. J. Iacono. 1981. Bacteriolysis of Streptococcus IoluttlIos BHT by lysozyme and inorganic anions normally present in human saliva. Arch. Oral Biol. 26:711-716. 24. Reider, J. L., and F. L. Macrina. 1977. Plasmid DNA isolation in Streptococcuts ot1iut(utns: glycine-enhanced ce...

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 1984

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.44.1.76-81.1984