Characterization of Mutations That Affect the Nonoxidative Pentose Phosphate Pathway in Sinorhizobium meliloti

Characterization of the -Ketoadipate Pathway in Sinorhizobium meliloti

Characterization of Sinorhizobium meliloti triose phosphate isomerase genes.

A Tn5 mutant strain of Sinorhizobium meliloti with an insertion in tpiA (systematic identifier SMc01023), a putative triose phosphate isomerase (TPI)-encoding gene, was isolated. The tpiA mutant grew more slowly than the wild type on rhamnose and did not grow with glycerol as a sole carbon source. The genome of S. meliloti wild-type Rm1021 contains a second predicted TPI-encoding gene, tpiB (SM...

The pentose phosphate pathway.

I received my basic training in enzymology as a graduate student in the laboratory of Professor T. R. Hogness at the University of Chicago from 1936 to 1939. Hogness had constructed a photoelectric spectrophotometer modeled after the one in Otto Warburg’s laboratory in Berlin-Dahlem. I was assigned a problem on succinic dehydrogenase from beef heart, using the Warburg manometric apparatus, and ...

Pentose phosphate pathway

5 Abbreviations 6PGD 6-phosphogluconate dehydrogenase ADP adenosine diphosphate ATP adenosine triphosphate CSF cerebrospinal fluid DHAP dihydroxyacetone phosphate G6PD glucose-6-phosphate dehydrogenase GA glyceraldehyde GAPDH glyceraldehyde-3-phosphate dehydrogenase GSG oxidized glutathione GSSG reduced glutathione LC-MS/MS liquid chromatography-tandem mass spectrometry MRI magnetic resonance i...

Characterization of the Rhizobium (Sinorhizobium) meliloti high- and low-affinity phosphate uptake systems.

Genetic studies have suggested that Rhizobium (Sinorhizobium) meliloti contains two distinct phosphate (Pi) transport systems, encoded by the phoCDET genes and the orfA-pit genes, respectively. Here we present data which show that the ABC-type PhoCDET system has a high affinity for Pi (Km, 0.2 microM) and that Pi uptake by this system is severely inhibited by phosphonates. This high-affinity up...