Characterization of Membrane-Associated Progesterone Receptor Component-2 (MAPRC2) from Trichinella spiralis and Its Interaction with Progesterone and Mifepristone

Trichinellosis is a foodborne zoonotic disease caused by Trichinella spp., including spiralis. In the present study, T. spiralis membrane-associated progesterone receptor component-2 (Ts-MAPRC2) gene was cloned and characterized using protein sequencing analysis. Furthermore, expression, purification, immunoblot assay, binding ability with antibody, immunofluorescence assay were performed. A direct effect of (P4) mifepristone (RU486) on Ts-MAPRC2 determined in vitro cell culture that showed different expression levels at all developmental stages (muscle larvae (ML), female adult worm (F-AL), male (M-AL), newborn (NBL)). Subsequently, phenotypic effects P4, RU486, rTs-MAPRC2-Ab F-AL ML measured. Later, vivo relative mRNA stage studied. Our results revealed critical to maintaining pregnancy (F-AL) The 300 ng/mL P4 100 RU486 downregulation (p ≤ 0.05). This plays an important role abortion possibly decreases burden host. Only 30 significant upregulation current study provides new insights regarding antihormone (P4 RU486) drug design vaccine therapy recombinant (rTs-MAPRC2) as well their combined control infection.