Characterization of genes associated with TGA7 during the floral transition

Abstract Background The TGACG-binding (TGA) family has 10 members that play vital roles in Arabidopsis thaliana defense responses and development. However, their involvement controlling flowering time remains largely unknown requires further investigation. Results To study the role of TGA7 during floral transition, we first investigated tga7 mutant, which displayed a delayed-flowering phenotype under both long-day short-day conditions. We then performed genetic pathway analysis found autonomous thermosensory pathways may affect expression. Furthermore, to reveal differential gene expression profiles between wild-type (WT) , cDNA libraries were generated for WT mutant seedlings at 9 days after germination. For each library, deep-sequencing produced approximately 6.67 Gb high-quality sequences, with majority (84.55 %) mRNAs being 500 3,000 nt. In total, 325 differentially expressed genes identified seedlings. Among them, four associated control. these flowering-related was validated by qRT-PCR. Conclusions control, FLC MAF5 be mainly responsible as regulated genes. These results provide framework studying promoting flowering.