Characterization of Family D DNA polymerase from Thermococcus sp. 9°N

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Pre-steady-state Kinetic Analysis of a Family D DNA Polymerase from Thermococcus sp. 9°N Reveals Mechanisms for Archaeal Genomic Replication and Maintenance*

Family D DNA polymerases (polDs) have been implicated as the major replicative polymerase in archaea, excluding the Crenarchaeota branch, and bear little sequence homology to other DNA polymerase families. Here we report a detailed kinetic analysis of nucleotide incorporation and exonuclease activity for a Family D DNA polymerase from Thermococcus sp. 9°N. Pre-steady-state single-turnover nucle...

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ژورنال

عنوان ژورنال: Extremophiles

سال: 2014

ISSN: 1431-0651,1433-4909

DOI: 10.1007/s00792-014-0646-9