Characterization of equine zona pellucida glycoproteins by polyacrylamide gel electrophoresis and immunological techniques
نویسندگان
چکیده
منابع مشابه
Characterization of human zona pellucida glycoproteins.
The human egg may only be fertilized by one spermatozoon to prevent polyploidy. In most mammals, the primary block to polyspermy occurs at the zona pellucida (ZP). Little is known of the human ZP and the changes occurring following fertilization to prevent polyploidy. Using antibodies directed against synthetic peptides predicted from the human ZP2 and ZP3 cDNA, we identified ZP3 as a 53-60 kDa...
متن کامل1 Zona Pellucida Glycoproteins
Abbreviations: ZP, zona pellucida; ZPG, ZP glycoprotein; VE, vitelline envelope; VEG, VE glycoprotein; mZP, mouse ZP; hZP, human ZP; SS, signal sequence; CTP, C-terminal propeptide; CFCS, consensus furin cleavage-site; TMD, transmembrane domain; ZPD, ZP domain; NTS, N-terminal subdomain; CTS, C-terminal subdomain; EHP, external hydrophobic patch; IHP, internal hydrophobic patch; CP, charged pat...
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Canine mycoplasmas which had been characterized by biological and serological methods were further studied by using polyacrylamide gel electrophoresis (PGE) and double diffusion in agar gel. The three dog mycoplasmas previously characterized, Mycoplasma canis, M. maculosum, and M. spumans showed distinctive patterns by PGE. Five additional representative isolates from dogs had been characterize...
متن کاملAcrosome reaction: relevance of zona pellucida glycoproteins.
During mammalian fertilisation, the zona pellucida (ZP) matrix surrounding the oocyte is responsible for the binding of the spermatozoa to the oocyte and induction of the acrosome reaction (AR) in the ZP-bound spermatozoon. The AR is crucial for the penetration of the ZP matrix by spermatozoa. The ZP matrix in mice is composed of three glycoproteins designated ZP1, ZP2 and ZP3, whereas in human...
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For many years the major problem impeding the elucidation of the structure of melanin and the melanosome has been the insolubility of the melanin polymer. To date, alkaline and acid hydrolysis seem to be the only methods available which can completely solubilize the melanosome (1); unfortunately, both methods degrade the constituents of the organelle other than the melanin polymer. The recent e...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1992
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0960815