Characterization of coagulase-positive Staphylococcus spp., antimicrobial resistance profile, and presence of enterotoxin-producing genes in goat milk in Paraná State

Goat milk presents interesting characteristics to consumers, but the production of this food faces several challenges that influence its quality. Process failures from milking processing and commercialization can expose contamination by pathogenic microorganisms, including coagulase-positive Staphylococcus group. Although aureus is most relevant species in mastitis poisoning, other group are also important, especially those related poisoning. This study aimed identify goat using biochemical tests, determine prevalence antimicrobial resistance disc diffusion test, investigate enterotoxin-producing genes, sea, seb, sec, sed, see, seg, seh, sei multiplex PCR. A total 384 strains obtained raw collected nine farms Paraná during four seasons year were studied. Biochemical tests showed 85.69% S. aureus, followed 9.38% intermedius, 4.17% hyicus 0.78% delphini. For up three each identified as Staphylococci chosen farm, with a 74 strains. Of these, 27% (20/74) at least one antibiotic, among all penicillin-resistant strains, 45% (9/20) presented tetracycline. In search for staphylococcal 49 studied; them, 40.81% (20/49) genes. The highest was detected sec gene, which present 22.44% (11/49) seh gene 18.36% (9/49), see 4.08% (2/49). It concluded predominant milk, high penicillin- tetracycline-resistant significant number studied carried genes involved poisoning humans, indicating possible source and, therefore, potential danger public health.