Characterization of cis-4-hydroxy-D-proline dehydrogenase from Sinorhizobium meliloti

Epimerization of trans-4-hydroxy-L-proline to cis-4-hydroxy-D-proline during acid hydrolysis of collagen.

1. trans-4-Hydroxy-r,-proline is epimerized to &+hydroxy-n-proline under the conditions generally used for the hydrolysis of proteins for analyses of amino acids. 2. The cis epimer elutes from a column of UR-30 resin on a Beckman amino acid analyzer at the same time as threonine and hence would be undetected. 3. About 8% of the trans-4-hydroxy-L-proline in collagen can be epimerized to c&4-hydr...

Isolation, Purification and Characterization of Proline Dehydrogenase from a Pseudomonas putida POS-F84 Isolate

The purpose of this study was to isolate and characterize Proline Dehydrogenase (ProDH) enzyme frommicroorganisms isolated from soil in Iran. Isolation and screening of L-proline degradative enzymes from soilsamples was carried out. The isolate was characterized by biochemical markers and 16S rRNA geneanalysis. The target ProDH was purified and the effects of pH and temperatur...

Characterization of Sinorhizobium meliloti triose phosphate isomerase genes.

A Tn5 mutant strain of Sinorhizobium meliloti with an insertion in tpiA (systematic identifier SMc01023), a putative triose phosphate isomerase (TPI)-encoding gene, was isolated. The tpiA mutant grew more slowly than the wild type on rhamnose and did not grow with glycerol as a sole carbon source. The genome of S. meliloti wild-type Rm1021 contains a second predicted TPI-encoding gene, tpiB (SM...

Characterization of the -Ketoadipate Pathway in Sinorhizobium meliloti

Conversion of C14-and cis-or trans-4-H3-L-proline to 4-hydroxy-L-proline and 4-oxo-L-proline by Streptomyces antibioticus.

26 Gerhart, J. C., and A. B. Pardee, in Synthesis and Structure of Macromolecules, Cold Spring Harbor Symposia on Quantitative Biology, vol. 28 (1963), p. 491. 27 Changeux, J. P., in Subunit Structure of Proteins, Brookhaven Symposia in Biology, no. 17 (1964), p. 232. 28 Antonini, E., J. Wyman, M. Brunori, J. F. Taylor, A. Rossi-Fanelli, and A. Caputo, J. Biol. Chem., 239, 907 (1964). 29 Gibson...