Characterization of Carnivorous Plants Sarracenia purpurea L. Transformed with Agrobacterium rhizogenes

People have used carnivorous plants of the genus Sarracenia in folk medicine for centuries due to biochemical composition plants, which are rich numerous bioactive compounds with anti-inflammatory, antioxidant, antiviral and antibacterial properties. The subject this study was genetic transformation purpurea L. Agrobacterium rhizogenes strains 15834, 9402 A4 using two different methods: bacterial injection or co-culture bacteria plant explants. This confirmed possibility hairy root induction S. A. strain 15834 method. Seven lines transformed exhibiting integration rolB gene, were obtained. roots formed showed morphological differences comparison unmodified plants. A mathematical model optimize conditions extraction compounds. Extracts isolated under optimal from changes, i.e., an increase accumulation total polyphenols (line 7#1 roots: 71.048 mg GAE g−1 DW; leaves: 9.662 DW) triterpenes 1.248 BA 0.463 untransformed (polyphenols 7.957 DW 5.091 0.298 0.296 DW), especially when induced analyzed. HPLC analysis level betulinic acid some lines. Betulinic is a pentacyclic triterpenoid compound high pharmacological significance.