Characterization of Brevibacillus laterosporus Cas9 (BlatCas9) for Mammalian Genome Editing
نویسندگان
چکیده
منابع مشابه
Genome Sequences of Five Additional Brevibacillus laterosporus Bacteriophages
Brevibacillus laterosporus has been isolated from many different environments, including beehives, and produces compounds that are toxic to many organisms. Five B. laterosporus phages have been isolated previously. Here, we announce five additional phages that infect this bacterium, including the first B. laterosporus siphoviruses to be discovered.
متن کاملGenome editing using Cas9 nickases.
The RNA-guided, sequence-specific endonuclease Cas9 has been widely adopted as genome engineering tool due to its efficiency and ease of use. Derived from the microbial CRISPR (clustered regularly interspaced short palindromic repeat) type II adaptive immune system, Cas9 has now been successfully engineered for genome editing applications in a variety of animal and plant species. To reduce pote...
متن کاملCorrection for Sheflo et al., Complete Genome Sequences of Five Brevibacillus laterosporus Bacteriophages
Volume 1, no. 6, e00668-13, 2013. The species infected by the five bacteriophages was misidentified as Paenibacillus larvae. The article title should read as given above, and the organism identified as Paenibacillus larvae should be identified as Brevibacillus laterosporus throughout. Page 1: The abstract should read as follows. “Brevibacillus laterosporus is a secondary invader following Europ...
متن کاملDraft Genome Sequence of Entomopathogenic Brevibacillus laterosporus Strain Lak 1210, an Alkaliphilic Chitin Degrader
We announce here the draft genome sequence of Brevibacillus laterosporus strain Lak 1210, isolated from mangrove soil. This alkaliphilic strain is an efficient chitin degrader and has the ability to control insects and inhibit phytopathogenic fungi. The assembly consists of 5,082,926 bp, with 4,321 protein-coding sequences and a GC content of 41.15%.
متن کاملCas9-based genome editing in zebrafish.
Genome editing using the Cas9 endonuclease of Streptococcus pyogenes has demonstrated unprecedented efficacy and facility in a wide variety of biological systems. In zebrafish, specifically, studies have shown that Cas9 can be directed to user-defined genomic target sites via synthetic guide RNAs, enabling random or homology-directed sequence alterations, long-range chromosomal deletions, simul...
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ژورنال
عنوان ژورنال: Frontiers in Cell and Developmental Biology
سال: 2020
ISSN: 2296-634X
DOI: 10.3389/fcell.2020.583164