Characterization of Biochemical Properties of Bacillus subtilis RecQ Helicase
نویسندگان
چکیده
منابع مشابه
Biochemical characterization of the DNA helicase activity of the escherichia coli RecQ helicase.
We demonstrate that RecQ helicase from Escherichia coli is a catalytic helicase whose activity depends on the concentration of ATP, free magnesium ion, and single-stranded DNA-binding (SSB) protein. Helicase activity is cooperative in ATP concentration, with an apparent S(0.5) value for ATP of 200 microm and a Hill coefficient of 3.3 +/- 0.3. Therefore, RecQ helicase utilizes multiple, interact...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2014
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.06367-11