Characterization of Adipose‐derived Stem Cells from Breast Cancer Cell Transplanted Mice

Mesenchymal stem cells (MCSs) from adipose tissue are reported to have a pronounced impact on tumor growth or suppression. However, so far, how the cancer affect in vivo conditions has not been clarified. In this study, we evaluated of breast characteristics adipose-derived (ADSCs) under conditions. ADSCs were obtained Balb/c mice three consecutive weeks after transplantation with 1×106 MCF7 cells. The results showed that amount per one gram fat reduced over time, but reduction was significantly different. fact, isolated presenting specific MSC surface markers increased at second passage, compared time isolation. Particularly, they highly positive for CD90 (a indicator) and negative CD45 hematopoietic marker). Moreover, vitro culture, successfully differentiated into adipocytes, which detected by Oil Red O staining. This present experiment gives initial assessments ADSC characteristics; however, further investigations necessary fully evaluate differences cell potency immunophenotype caused transplantation.