Characterization of a Polyclonal Antihapten Antibody by Affinity Capillary Electrophoresis
نویسندگان
چکیده
منابع مشابه
Multiple-Injection Affinity Capillary Electrophoresis
Please address correspondence to Frank A. Gomez at [email protected]. nteractions between biological species are essential to life and are involved directly in many of the enzyme-based reactions involved in cell division, cell death, and cell transformations. These biological interactions are important in the initiation, progression, and harmful effects of human disease including Parkinson...
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Partial-filling affinity capillary electrophoresis (PFACE) is used to examine the binding interactions between two model biological systems: D-Ala-D-Ala terminus peptides to the glycopeptide antibiotic vancomycin (Van) from Streptomyces orientalis, and arylsulfonamides to carbonic anhydrase B (CAB, EC 4.2.1.1, bovine erythrocytes). Using these two systems, modifications in the PFACE technique a...
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Papers detailing the use of affinity capillary electrophoresis (ACE) in examining binding parameters between biological species are summarized in this review. The works cited in this review were published during 2007 and were drawn from the major chemical and biochemical journals and especially the major analytical chemistry literature.
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Lipid hydroperoxide may react with protein or amino phospholipid without secondary decomposition. We prepared a polyclonal an tibody to lipid hydroperoxide-modified proteins using 13S-hydroperoxy-9Z, 1 1E-octadecadienoic acid-modified keyhole limpet hemocyanin (13-HPODEKLH) as immunogen. The antibody recognized 13-HPODEmodified bovine serum albumin (BSA), but not aldehydemodified proteins, such...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2000
ISSN: 0003-2697
DOI: 10.1006/abio.2000.4747