Characterization of a Gene Encoding DNA Repair Protein XRCC3 from Arabidopsis thaliana

H-Protein of the Glycine Decarboxylase Multienzyme Complex : Complementary DNA Encoding the Protein from Arabidopsis thaliana.

کلن کردن ژنRGL2 از DNA ژنومی آرابیدوپسیس (Arabidopsis thaliana)

یکی از ژنهایی که در سنتز جیبرلین در گیاه شناخته شده و موقعیت آنها در ژنوم آرابیدپسیس مشخص شده است، RGL2 می‌باشد که در این بررسی کلن گردید. با استخراج DNA از آرابیدوپسیس، اکوتیپ کلمبیا و استفاده از آغازگرهای اختصاصی که بر اساس توالی ژن مورد نظر ساخته شده بودند، این ژن تکثیر شد. پلاسمید  PET-32a (+)و ژن تکثیر شده با استفاده از دو آنزیم برشی خاص به نامهای Nco I و Sal I برش داده شدند. پس از اتصال ژ...

Expression, purification, and characterization of the protein repair l-isoaspartyl methyltransferase from Arabidopsis thaliana.

Protein l-isoaspartate (d-aspartate) O-methyltransferase (EC 2.1.1. 77) is a repair enzyme that methylates abnormal l-isoaspartate residues in proteins which arise spontaneously as a result of aging. This enzyme initiates their conversion back into the normal l-aspartate form by a methyl esterification reaction. Previously, partial cDNAs of this enzyme were isolated from the higher plant Arabid...

Protein expression and characterization of SEP3 from Arabidopsis thaliana.

SEPALLATA (SEP) MADS-box genes play crucial roles in the regulation of floral growth and development. They are required for the specification of sepals, petals, stamens, and carpels as well as for floral determinacy. SEPs perform their functions through the formation of homo- or hetero-polymers, which are the molecular basis of floral quartets. In vitro assays indicated that SEP3 forms a tetram...

Genomic nucleotide sequence of a gene from Arabidopsis thaliana encoding a protein homolog of Escherichia coli RecA.

In Escherichia coli and many other prokaryotes, the RecA protein is essential for homologous recombination and for a variety of S.O.S. responses to DNA damage (Little and Mount, 1982; Kowalczykowski, 1991). Previously, Cerutti et al. (1992) found a chloroplast-localized strand recombination activity resembling that of bacterial RecA and a protein band cross-reacting with antibody to E. coli Rec...