Characterization and Performance of Lactate-Feeding Consortia for Reductive Dechlorination of Trichloroethene

Understanding the underlying mechanism that drives microbial community mediated by substrates is crucial to enhance biostimulation in trichloroethene (TCE)-contaminated sites. Here, we investigated performance of stable TCE-dechlorinating consortia monitoring variations TCE-related metabolites and explored their assembly mechanisms using 16S rDNA amplicon sequencing bioinformatics analyses. The results indicated three were successfully enriched lactate-containing anaerobic media. statistical analysis demonstrated communities enrichment cultures changed along with time distinguished sample sources. deterministic stochastic processes simultaneously responsible for shaping assembly. indicator patterns shifted exhaustion carbon source pollutants, tceA-carrying Dehalococcoides, as an final stage samples, responded positively TCE removal during incubation period. Pseudomonas, Desulforhabdus, Desulfovibrio Methanofollis identified keystone populations process co-occurrence network analysis. this study indicate lactate can be effective substrate stimulated bioremediation TCE-contaminated sites, reduction forces or enhancement interventions may promote more biostimulation.