Characterization and identification of Trichoderma on shallots isolated from three elevation regions in West Sumatra, Indonesia

Abstract. Susila E, Maulina F, Emilda D. 2023. Characterization and identification of Trichoderma on shallots isolated from three elevation regions in West Sumatra, Indonesia. Biodiversitas 24: 2064-2071. Various microorganisms are found the rhizosphere plants. The species exhibited many benefits to plants such as biological control crop diseases their causing agents. This study aim was explore, collect, identify shallot based morphological characters molecular techniques. research carried out March October 2021 at Biology Laboratories Agricultural State Polytechnic Payakumbuh Plant Protection Laboratory, Indonesian Tropical Fruits Research Institute (ITFRI) for observation, Molecular Laboratories, ITFRI identification. Samples were collected Alahan Panjang (1700 m above sea level), Solok (400m asl) Kambang (< 200 by using Stratified Random Sampling method. isolates cultured Potato Dextose Agar (PDA). Three sp. obtained identified. Four specific primer pairs (T2A F-T2A R, T2 F-T2 T1 F-T1 R Th1 F-Th1 R) used amplification results spp. four primers showed that tested amplified only T. asperellum pair. origin locations (Alahan Panjang, Kambang) is identified one i.e. asperellum.