Characterization and applications of a monoclonal antibody against infectious salmon anaemia virus
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چکیده
منابع مشابه
Characterization and applications of a monoclonal antibody against infectious salmon anaemia virus.
The preparation of the first monoclonal antibody (MAb) against the orthomyxovirus-like infectious salmon anaemia (ISA) virus is described. Characterization of the MAb included isotyping, enzyme-linked immunosorbent assay (ELISA), immunofluorescent staining of virus infected cell cultures (SHK-1 cells), immunoelectron microscopy (IEM) of negatively stained virus preparations, virus neutralizatio...
متن کاملGenomic organization of infectious salmon anaemia virus.
The RNA genome segment order, nucleotide sequence and the putative encoded proteins were determined for infectious salmon anaemia virus (ISAV). Eight segments of genomic viral RNA between 1.0 and 2.4 kb in length were identified. RNA segments 1-6 each had a predicted single open reading frame encoding the P1, PB1, NP, P2, P3 and HA proteins, respectively. Segment 7 encoded the P4/P5 proteins an...
متن کاملRelative resistance of Pacific salmon to infectious salmon anaemia virus.
Infectious salmon anaemia (ISA) is a major disease of Atlantic salmon, Salmo salar, caused by an orthomyxovirus (ISAV). Increases in global aquaculture and the international movement of fish made it important to determine if Pacific salmon are at risk. Steelhead trout, Oncorhynchus mykiss, and chum, O. keta, Chinook, O. tshawytscha, coho, O. kisutch, and Atlantic salmon were injected intraperit...
متن کاملInfectious salmon anaemia virus (ISAV) mucosal infection in Atlantic salmon
All viruses infecting fish must cross the surface mucosal barrier to successfully enter a host. Infectious salmon anaemia virus (ISAV), the causative agent of the economically important infectious salmon anaemia (ISA) in Atlantic salmon, Salmo salar L., has been shown to use the gills as its entry point. However, other entry ports have not been investigated despite the expression of virus recep...
متن کاملCloning and identification of the infectious salmon anaemia virus haemagglutinin.
Infectious salmon anaemia virus (ISAV) is an orthomyxo-like virus that causes serious disease in Atlantic salmon (Salmo salar). Like the orthomyxoviruses, ISAV has been shown to possess haemagglutinin (HA) activity. This study presents the cloning, expression and identification of the ISAV HA gene, which was isolated from a cDNA library by immunoscreening. The HA gene contained an ISAV-specific...
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ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 1998
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao034077