Characterisation of Macrophage Inhibitory Factor-2 (MIF-2) in Haemonchus contortus and Teladorsagia circumcincta

Full-length cDNAs encoding macrophage inhibitory factor-2 (MIF-2) were cloned from Teladorsagia circumcincta (TcMIF-2) and Haemonchus contortus (HcMIF-2). TcMIF-2 HcMIF-2 cDNA (342 bp) encoded proteins of 114 amino acids, each which was present as a single band about 16 kDa on SDS-PAGE. Multiple alignments the protein sequences showed homology 84% between HcMIF-2, 54–76% with MIF-2s seven nematodes, but low other MIF sequences. The predicted three-dimensional structures revealed an overall structural highly conserved binding catalytic sites minor differences in tautomerase site residues nematode MIF-2 homologues. A phylogenetic tree constructed using helminth mammalian MIF-1 Soluble C-terminal arabinose inducible promotor AY2.4, expressed Escherichia coli strain AY2.4 purified. Recombinant had similar enzyme activities standard assay. activity approximately halved by storage at 4 °C, −20 °C or −70 °C. Antibodies serum saliva field-immune, not nematode-naïve, sheep recognised recombinant enzyme-linked immunosorbent assays. Recognition antibodies generated exposure to native indicates antigenicity two proteins.