منابع مشابه
Cerebroside galactosidase of brain.
The galactoside bond in cerebroside was found to be cleaved by an enzyme in rat and pig brain. Emulsified stearoyl-(14)C psychosine was used as the substrate and the extent of cleavage was studied by isolating and counting the stearoyl sphingosine (ceramide) formed. The reaction products, ceramide and galactose, were characterized by column and thin-layer chromatography. Cerebroside containing ...
متن کاملPurification of cerebroside galactosidase from rat brain.
A method is described for the preparation of a B-galactosidase which acts on the brain lipid, ceramide galactoside. The enzyme is obtained from particles that sediment between I 000-13 ooo xg from rat brains and is extracted with a 60,& sodium cholate solution. The enzyme is solubilized and inactive proteins removed by digestion with pancreatic enzymes and the cholate is removed by dialysis and...
متن کاملProperties of cerebroside galactosidase.
Rat brain cerebrosidase was shown by centrifugal fractionation to be a lysosomal enzyme. The pH optimum was found to be 4.5. Taurocholate greatly stimulated its action. The addition of palmitic acid had little effect on the enzyme activity, but ceramide, sphingosine, and ceramide lactoside inhibited. Galactose, galactonolactone, and galactitol inhibited the enzyme, but the corresponding compoun...
متن کاملCeramide-like synthetic amides that inhibit cerebroside galactosidase.
Amides resembling ceramide (fatty acyl sphingosine) were synthesized and tested for their effects on rat brain cerebrosidase (galactosyl ceramide beta-galactosidase). The best inhibitor was N-decanoyl dl-erythro-3-phenyl-2-aminopro-panediol, which exhibited a K(i) of 0.4 mm. A Lineweaver-Burk plot indicated that the amide acted as a noncompetitive inhibitor, presumably by attachment to a site o...
متن کاملCharacterization of cerebroside sulfotransferase from rat brain.
Cerebroside sulfotransferase, the enzyme which catalyzes the transfer of the sulfate group from d’-phosphoadenosine 5’-phosphosulfate to galactosylceramide to form sulfogalactosylceramide is located primarily in microsomes and possibly in myelin of 17-day-old rat brain. Subfractionation of the microsomes demonstrates the enzyme activity to be present in the lighter, smooth membrane fraction. Vi...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1966
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)38965-3