Ceramide metabolite, not intact ceramide molecule, may be responsible for cellular toxicity
نویسندگان
چکیده
منابع مشابه
Ceramide metabolite, not intact ceramide molecule, may be responsible for cellular toxicity.
Ceramides, which are produced from the hydrolysis of sphingomyelin or synthesized from serine and palmitate in a de novo pathway, are regarded as important cellular signals for inducing apoptosis. However, controversy over this proposed role of ceramides exists. Using stable isotope labelling coupled with GC (gas chromatography)-MS and mass isotopomer distribution analysis, we have studied the ...
متن کاملMechanisms involved in cellular ceramide homeostasis
Sphingolipids are ubiquitous and critical components of biological membranes. Their biosynthesis starts with soluble precursors in the endoplasmic reticulum and culminates in the Golgi complex and plasma membrane. Ceramides are important intermediates in the biosynthesis of sphingolipids, such as sphingomyelin, and their overload in the membranes is injurious to cells. The major product of cera...
متن کاملCeramide glycosylation potentiates cellular multidrug resistance.
Ceramide glycosylation, through glucosylceramide synthase (GCS), allows cellular escape from ceramide-induced programmed cell death. This glycosylation event confers cancer cell resistance to cytotoxic anticancer agents [Liu, Y. Y., Han, T. Y., Giuliano, A. E., and M. C. Cabot. (1999) J. Biol. Chem. 274, 1140-1146]. We previously found that glucosylceramide, the glycosylated form of ceramide, a...
متن کاملCeramide kinase and ceramide-1-phosphate.
It has been over a decade since the sphingolipid ceramide-1-phosphate (C1P) was described. Until recently, only sparse reports on possible biological functions for this lipid have been published. A large number of reports have now surfaced demonstrating distinct biological mechanisms regulated by C1P produced from ceramide kinase (CERK). In the following methods chapter, the methodologies for e...
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Ceramide kinase (CERK) is a critical mediator of eicosanoid synthesis, and its product, ceramide-1-phosphate (C1P), is required for the production of prostaglandins in response to several inflammatory agonists. In this study, mass spectrometry analysis disclosed that the main forms of C1P in cells were C(16:0) C1P and C(18:0) C1P, suggesting that CERK uses ceramide transported to the trans-Golg...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 2004
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj20031733