Cellular Transmission of Mouse Sarcomata with Frozen-dried Tumour Tissues

Skeletal sarcomata and giant-cell tumour.

There is no universal agreement on a classification of bone tumours. Iany of the existing methods are based on varying criteria and consequently the nomenclature is confused. Numerous descriptive terms are employed to denote only minor and irrelevant differences in essentially similar tumours. If a classification is to be more than an academic exercise, the diagnosis of a particular type of tum...

Electron Microscope Observations on Frozen-dried Cells

To explore the problem of artefacts which may be produced during usual fixation, dehydration, and embedding, the authors have examined pancreas, liver, and bone marrow frozen at about -180 degrees C., dried, at -55 to -60 degrees C., embedded in methacrylate, sectioned, and floated on a formol-alcohol mixture. By these treatments the labile structure of living cells can be fixed promptly and em...

Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa.

Chromosome stability was maintained in mouse spermatozoa after freeze-drying or freezing without cryoprotection in a simple Tris.HCl buffer containing EGTA (50 mM) and NaCl (50 mM). The ability of spermatozoa to activate oocytes spontaneously was not destroyed by freeze-drying or freezing without cryoprotection in this solution. Embryos derived after injecting oocytes with sperm heads from rehy...

The Surgery of Sarcomata

Effect of oral co-administration of frozen-dried grapefruit juice on pharmacokinetics of tramadol in dogs

Tramadol is a centrally acting analgesic drug extensively metabolized in animal species. Its clinicalresponse is mainly due to the M1 metabolite, poorly produced in dogs. Grapefruit-juice can inhibit themetabolism of different drugs in animals and humans. The aim of the present study was to evaluate thepharmacokinetics of tramadol and its major metabolites after co-administration of tramadol an...