Cellular Swelling

The Cellular Mechanisms of Neuronal Swelling Underlying Cytotoxic Edema

Cytotoxic brain edema triggered by neuronal swelling is the chief cause of mortality following brain trauma and cerebral infarct. Using fluorescence lifetime imaging to analyze contributions of intracellular ionic changes in brain slices, we find that intense Na(+) entry triggers a secondary increase in intracellular Cl(-) that is required for neuronal swelling and death. Pharmacological and si...

Reproducibility after de-swelling and re-swelling

Mechanism of Cellular Swelling Induced by Extracellular Lactic Acidosis in Neuroblastoma-Glioma Hybrid

The mechanism of cellular swelling induced by extracellular lactic acidosis and the effect of diuretics were studied using neuroblastoma-glioma hybrid (NG10815) cells. The cells were incubated in one of three lactate concentrations (0,15, or 30 mM), each of which was randomized to one of three pH groups (7.4, 6.2, or 5.0). Analysis of the swelling was measured using a Coulter counter technique....

Decreased ADC precedes cellular swelling in N-Methyl-D-Aspartate (NMDA) treated mouse retina

Introduction Acute excitotoxicity causes cytotoxic edema that precedes neuron cell necrosis and apoptosis in neurological diseases. Apparent diffusion coefficient (ADC) decreases early reflecting cytotoxic edema in various brain injuries. Our recent study demonstrated that high-resolution diffusion MRI detected three retinal layers of distinct ADC. In this study, we examined the feasibility of ...

Mechanism of cellular swelling induced by extracellular lactic acidosis in neuroblastoma-glioma hybrid (NG108-15) cells.

The mechanism of cellular swelling induced by extra-cellular lactic acidosis and the effect of diuretics were studied using neuroblastoma-glioma hybrid (NG108-15) cells. The cells were incubated in one of three lactate concentrations (0, 15, or 30 mM), each of which was randomized to one of three pH groups (7.4, 6.2, or 5.0). Analysis of the swelling was measured using a Coulter counter techniq...