Cell-free translation of murine coronavirus RNA
نویسندگان
چکیده
منابع مشابه
Cell-free translation of paramyxovirus messenger RNA.
Polypeptides corresponding in electrophoretic mobility to virion polypeptides 1, 3, and 5 were made in a reticulocyte cell-free system to which 18S RNA from Sendai virus-infected cells was added. Immune precipitation was used to select relevant polypeptides from endogenous products. The cell-free product corresponding to virion polypeptide 3 (the nucleocapsid structure unit) was the most abunda...
متن کاملTranslation of murine leukemia virus RNA in cell-free systems from animal cells.
The virion RNA of Moloney murine leukemia virus (MuLV) has been translated in eukaryotic cell-free systems derived from mouse L- and human HeLa cells. In both systems at least three polypeptides, approximately 60,000, 70,000, and 180,000 in apparent molecular weight, were formed in response to the added 35S MuLV RNA. All three polypeptides were precipitable with antiserum to detergent-disrupted...
متن کاملCell free translation of chicken calcitonin messenger RNA.
The primary step of calcitonin biosynthesis was studied in a normal organ: chicken ultimobranchial gland, a tissue particularly rich in calcitonin secretory cells. Poly(A)-rich RNA was extracted and purified from ultimobranchial organs and translated in a reticulocyte lysate in the presence of labelled methionine. Polyacrylamide gel electrophoresis of specific immunoprecipitates revealed a majo...
متن کاملCell-free translation of highly purified adenovirus messenger RNA.
Several polypeptides contained in the coat and the core of adenovirus type 2 particles have been identified as virus gene products. They are synthesized in vitro by a cell-free protein-making system programmed by adenovirus type 2 messenger RNA that has been hybridized with and eluted from virus DNA. Fractionation by size yields subpopulations of viral messenger RNA that differ in their coding ...
متن کاملFunctional analysis of the murine coronavirus genomic RNA packaging signal.
Coronaviruses selectively package genomic RNA into assembled virions, despite the great molar excess of subgenomic RNA species that is present in infected cells. The genomic packaging signal (PS) for the coronavirus mouse hepatitis virus (MHV) was originally identified as an element that conferred packaging capability to defective interfering RNAs. The MHV PS is an RNA structure that maps to th...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1982
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.43.3.905-913.1982