Cell free protein synthesis: a viable option for stratified medicines manufacturing?

A picoliter chamber array for cell-free protein synthesis.

The completion of human genome sequencing has shifted the focus of research from genes to proteins. In this regard, a protein library chip has become a useful tool for cell-free protein synthesis. In this study, we attempted to make a highly-integrated protein chip from a DNA library using in vitro protein synthesis on a microchamber array fabricated by using PDMS (polydimethyl siloxane), a hyd...

A cell-free protein synthesis system for high-throughput proteomics.

We report a cell-free system for the high-throughput synthesis and screening of gene products. The system, based on the eukaryotic translation apparatus of wheat seeds, has significant advantages over other commonly used cell-free expression systems. To maximize the yield and throughput of the system, we optimized the mRNA UTRs, designed an expression vector for large-scale protein production, ...

Creating a completely "cell-free" system for protein synthesis.

Cell-free protein synthesis is a promising tool to take biotechnology outside of the cell. A cell-free approach provides distinct advantages over in vivo systems including open access to the reaction environment and direct control over all chemical components for facile optimization and synthetic biology integration. Promising applications of cell-free systems include portable diagnostics, biot...

biofeedback for the treatment of stress urinary incontinence: a viable option for select patients

Oral health researcher: a viable career option.

Encouraging individuals to pursue a career in oral health research is crucial if the Canadian dental profession wishes to continue moving its collective knowledge base forward. As dentistry increasingly moves toward an evidence-based model of delivery of care, members of the profession must engage in cutting-edge research. While the oral health research community in � Canada has rightfully earn...