Cell Free Fetal DNA: Marker for Predicting Pregnancy Outcomes

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Introducing a New and Simple Protocol for Capillary Electrophoresis of Cell Free Fetal Double Stranded DNA

Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we propos...

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Cell‑free fetal DNA (cff‑DNA) is a novel promising biomarker that has been applied in various aspects of obstetrical research, notably in prenatal diagnosis and complicated pregnancies. It is easily detected by semi‑quantitative PCR for the SRY target gene. It is well recognized that the levels of circulating cff‑DNA play a role in various complications of pregnancy. In this review, we explore ...

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Fetal RHD Genotyping Using Real-Time Polymerase Chain Reaction Analysis of Cell-Free Fetal DNA in Pregnancy of RhD Negative Women in South of Iran

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ژورنال

عنوان ژورنال: Indian Journal of Clinical Biochemistry

سال: 2017

ISSN: 0970-1915,0974-0422

DOI: 10.1007/s12291-017-0672-3