Cell Culture Method using Collagen Gel as Matrix
نویسندگان
چکیده
منابع مشابه
Unilocular fat cells in three-dimensional collagen gel matrix culture.
Three-dimensional culture with collagen gel, developed recently for the in vitro study of some mammalian cells in a more physiological condition than a monolayer culture, was applied for a biological study of unilocular fat cells. Successfully embedded in the gel, the unilocular fat cells were shown to be able to keep their cellular function and actively proliferate. These findings confirm that...
متن کامل[Efficacy of the chemosensitivity test using collagen gel matrix-supported culture system for urogenital tumors].
BACKGROUND We evaluated the usefulness of in vitro tumor culture system using a specialized collagen gel matrix (CGM assay) as a chemosensitivity test. PATIENTS AND METHODS Chemosensitivity results of CGM assay were compared with other in vivo and in vitro assays on an implantable murine bladder tumor cell line (MBT-2). In addition we investigated the possibility of the clinical use of CGM as...
متن کاملCulture of rabbit meibomian gland using collagen gel.
Rabbit whole meibomian glands were isolated by microsurgical dissection and enzymatic digestion. Both ductal and acinar elements could be separated by subsequent microsurgical manipulation. Epithelial outgrowth could be derived from either the whole gland or the ductal/acinar elements using collagen gel in the serum-containing, growth factor-enriched medium. Two types of outgrowths were observe...
متن کاملEvaluation of Human Breast Adenocarcinoma (MCF-7) Cells Proliferation in Co-Culture with Human Adipocytes in Three Dimensional Collagen Gel Matrix: Norepinephrine as a Lipolytic Factor
Background: Norepinephrine plays a trophic role in the control of cell replication and differentiation in target cells that express adrenergic receptors. Methods: In this study, we have tested the influence of infraphysiological, physiological and supraphysiological concentrations (0.0001 nM, 1 nM, 10000 nM) of human norepinephrine on the proliferation of breast cancer cells (human breast adeno...
متن کاملA concept for miniaturized 3-D cell culture using an extracellular matrix gel.
This paper presents a novel method to embed, anchor, and cultivate cells in a controlled 3-D flow-through microenvironment. This is realized using an etched silicon pillar flow chamber filled with extracellular matrix (ECM) gel mixed with cells. At 4 degrees C, while in liquid form, ECM gel is mixed with cells and injected into the chamber. Raising the temperature to 37 degrees C results in a g...
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ژورنال
عنوان ژورنال: Sen'i Gakkaishi
سال: 1986
ISSN: 0037-9875,1884-2259
DOI: 10.2115/fiber.42.6_p222