Cell culture media analysis using rapid spectroscopic methods

Analysis Using Infrared Spectroscopic Methods

Monitoring Cell Culture Media Degradation using Surface

Rapid quantification of tryptophan and tyrosine in chemically defined cell culture media using fluorescence spectroscopy.

The rapid and inexpensive analysis of the complex cell culture media used in industrial mammalian cell culture is required for quality and variance monitoring. Excitation-emission matrix (EEM) spectroscopy combined with multi-way chemometrics is a robust methodology applicable for the analysis of raw materials, media, and bioprocess broths. We have shown that the methodology can identify compos...

Rapid Characterisation and Quality Control of Complex Cell Culture Media Solutions using Raman Spectroscopy

The use of Raman spectroscopy coupled with chemometrics for the rapid identification, characterisation, and quality assessment of complex cell culture media components used for industrial mammalian cell culture was investigated. Raman spectroscopy offers significant advantages for the analysis of complex, aqueous based materials used in biotechnology because there is no need for sample preparat...

Spectroscopic conductivity imaging of a cell culture

In this paper, we present a simplified electrical model for tissue culture. We derive a mathematical structure for overall electrical properties of the culture and study their dependence on the frequency of the current. We introduce a method for recovering the microscopic properties of the cell culture from the spectral measurements of the effective conductivity. Numerical examples are provided...