CD133 positive progenitor endothelial cell lines from human cord blood
نویسندگان
چکیده
منابع مشابه
Hemogenic endothelial progenitor cells isolated from human umbilical cord blood.
Hemogenic endothelium has been identified in embryonic dorsal aorta and in tissues generated from mouse embryonic stem cells, but to date there is no evidence for such bipotential cells in postnatal tissues or blood. Here we identify a cell population from human umbilical cord blood that gives rise to both endothelial cells and hematopoietic progenitors in vitro. Cord blood CD34+/CD133+ cells p...
متن کاملcharacterization of megakaryocyte progenitor cells differentiated from umbilical cord blood cd133+ and cd133– cells
objective: this study was performed to determine the immunophenotype of megakaryocyte progenitor cells differentiated from ucb cd133+ and cd133− cells under the effects of interleukin–3 (il–3), interleukin–6 (il–6), stem cell factor (scf) and thrombopoietin (tpo) in vitro. materials and methods: cd133+and cd133− cells were isolated by using cd133 isolation kit following the manufacturer’s instr...
متن کاملComparison between Total Endothelial Progenitor Cell Isolation versus Enriched Cd133 Positive Culture
Endothelial progenitor cells (EPCs) play a role in endogenous neovascularization of ischemic tissues. Isolation and characterization of EPCs from circulating mononuclear cells is important for developing targeted cellular therapies and reproducibility of data is the major scientific goals. Here we compared two currently employed isolation methods, i.e. from total peripheral blood mononuclear ce...
متن کاملCD133 positive endothelial progenitor cells contribute to the tumour vasculature in non-small cell lung cancer.
AIMS Recent results generated in a mouse model suggest that tumour angiogenesis/vasculogenesis can be initiated and maintained by bone marrow derived endothelial progenitor cells. This present study investigated the distribution and frequency of CD133 positive endothelial progenitor cells in patients with non-small cell lung cancer (NSCLC) (tumour tissue and tumour free lung regions) and health...
متن کاملEndothelial progenitor cell culture and differentiation in vitro: a methodological comparison using human umbilical cord blood.
OBJECTIVE Endothelial progenitor cells (EPC) can contribute to vascular repair and targeted tumour therapy. Little is known about generating EPC from human umbilical cord blood. We therefore compared methods for purification of EPC from human umbilical cord blood. METHODS Mononuclear cells were isolated from human umbilical cord blood by density gradient centrifugation and used either unselec...
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ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2011
ISSN: 1552-4922
DOI: 10.1002/cyto.a.21092