Cation-exchange solid-phase and liquid-liquid extraction for the determination of khat alkaloids by reversed phase HPLC-DAD
نویسندگان
چکیده
منابع مشابه
Reversed-phase Salt Assisted Liquid-liquid Extraction: A New Technique for Preconcentration and Determination of Crocin in Herbal Medicines by HPLC
A reversed-phase salt assisted liquid-liquid extraction (RP-SALLE) method was developed for the preconcentration and determination of trace crocin in herbal medicines by high-performance liquid chromatography (HPLC). In developed method, the roles of the aqueous and organic phases are inverted so that the analyte is extracted from the organic into the aqueous phase after salt addition and phase...
متن کاملPreparative HPLC for large scale isolation, and salting-out assisted liquid–liquid extraction based method for HPLC–DAD determination of khat (Catha edulis Forsk) alkaloids
BACKGROUND Khat (Catha edulis Forsk) is an evergreen shrub of the Celastraceae family. It is widely cultivated in Yemen and East Africa, where its fresh leaves are habitually chewed for their momentary pleasures and stimulation as amphetamine-like effects. The main psychostimulant constituents of khat are the phenylpropylamino alkaloids: cathinone, cathine and norephedrine. RESULTS In this st...
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In the current study, injectable formulations containing Doxercalciferol as the active pharmaceutical ingredient are analyzed by using gradient-elution high-performance liquid chromatography with ultraviolet detection. Various related impurities and degradants are quantified by using solid-phase extraction (SPE) for enhanced sensitivity. The assay of possible related impurities and Doxercalcife...
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Primary aromatic amines (PAAs) have been broadly studied due to their high toxicity. In this work a method for the analysis of 22 PAAs in aqueous simulants has been developed. The method is based on a solid-phase extraction step using cation-exchange cartridges and the subsequent analysis of the extracts by ultra-high-performance liquid chromatography with mass spectrometric detection. The reco...
متن کاملDetermination of Alkaloids in Goldenseal Using Agilent Bond Elut Plexa Solid Phase Extraction Sorbent for Cleanup and HPLC-DAD Analysis
The alkaloids (hydrastine and berberine) in goldenseal were successfully cleaned up using the Bond Elut Plexa solid phase extraction sorbent. Their HPLC separation was achieved in less than 6 minutes employing an Agilent RRHD ZORBAX Eclipse Plus C18 column (4.6 x 75 mm, 3.5 μm) at 35 °C with gradient elution of 0.1% phosphoric acid/methanol mobile phase. The calibration curves of both alkaloids...
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ژورنال
عنوان ژورنال: Bulletin of the Chemical Society of Ethiopia
سال: 2015
ISSN: 1011-3924
DOI: 10.4314/bcse.v29i3.1