CaT1 knock-down strategies fail to affect CRAC channels in mucosal-type mast cells

(CRAC) Channels in Human T Cells

Although the crucial role of Ca 2 1 influx in lymphocyte activation has been well documented, little is known about the properties or expression levels of Ca 2 1 channels in normal human T lymphocytes. The use of Na 1 as the permeant ion in divalent-free solution permitted Ca 2 1 release-activated Ca 2 1 (CRAC) channel activation, kinetic properties, and functional expression levels to be inves...

Rapid conditional knock-down–knock-in system for mammalian cells

RNA interference (RNAi) is a powerful tool to analyze gene function in mammalian cells. However, the interpretation of RNAi knock-down phenotypes can be hampered by off-target effects or compound phenotypes, as many proteins combine multiple functions within one molecule and coordinate the assembly of multimolecular complexes. Replacing the endogenous protein with ectopic wild-type or mutant fo...

Distinct Properties of CRAC and MIC Channels in RBL Cells

In rat basophilic leukemia (RBL) cells and Jurkat T cells, Ca(2+) release-activated Ca(2+) (CRAC) channels open in response to passive Ca(2+) store depletion. Inwardly rectifying CRAC channels admit monovalent cations when external divalent ions are removed. Removal of internal Mg(2+) exposes an outwardly rectifying current (Mg(2+)-inhibited cation [MIC]) that also admits monovalent cations whe...

Populations of Mast Cells Agonist Elicits Graded Responses in All-or-None Activation of CRAC Channels by

Prime-Boost Strategies in Mucosal Immunization Affect Local IgA Production and the Type of Th Response

Combinations of different delivery routes for priming and boosting represent vaccination strategies that can modulate magnitude, quality, and localization of the immune response. A murine model was used to study T cell clonal expansion following intranasal (IN) or subcutaneous (SC) priming, and secondary immune responses after boosting by either homologous or heterologous routes. T cell primary...