Cascade Biocatalytic Processes through Combined Cross-linked Enzyme Aggregates

Cascade reactions catalyzed by immobilized multi enzymatic systems are emerging as a tool to address the increasing need for green and sustainable chemistry. In this regard, carrier-free immobilization strategy of combined cross-linked enzymes aggregates (combi- CLEAs) is great interest. combi-CLEAs two or more co-immobilized into single unit where they accommodated in close proximity order minimize intermediates diffusion reaction medium, at same time allowing access substrates coenzymes active sites. The use CLEAs provides simple low cost methodology that enhances operational storage enzyme stability facilitates biocatalyst recovery recycle. Some significant applications cascade combi- reported mini-review. These examples have been applied on multigram scale furnished practical steps towards implementation an industrial scale.