CARTILAGE MATRIX DEPLETION BY RHEUMATOID SYNOVIAL CELLS IN TISSUE CULTURE
نویسندگان
چکیده
منابع مشابه
Cartilage Matrix Depletion by Rheumatoid Synovial Cells in Tissue Culture
Articular cartilage fragments were added to monolayer cultures of synovial membrane cells. After 3 wk of incubation, the cartilage fragments were examined histologically for metachromasia and basophilia, and for fluorescent staining using a rabbit antiserum to cartilage protein-polysaccharide. Cartilage incubated with cells derived from rheumatoid synovial membranes showed striking loss of meta...
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EA R o CAL s-ruDis of normal and rheumatoid synovial membranes showed markedly increased acid phosphatase and oxidase activities in the lining cells of the rheumatoid membrane.' Acid phosphatase activity, evident in cytoplasmic granules, serves as a marker for lysosomes-subcellular organelles first described by deDuve and shown to contain numerous hydrolases active at an acid pH.2'3 Homogenates...
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Cultures of synovial cells obtained at surgery from patients with rheumatoid arthritis produced a protein which inhibits the mitosis of normal rabbit and human synovial cells, and of human lung fibroblasts. This mitotic inhibitory effect can be transferred to rabbit synovial cells by intra-articular injection of the animals with rheumatoid synovial cell cultures.
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A previous report described a cell isolate presumed to have arisen by accidental cocultivation (contamination) of the Chang 'liver' cell line and rheumatoid synovial cells. This cell isolate had the same glucose-6-phosphate dehydrogenase isoenzyme as the Chang cell and also some shared antigens. It clearly differed in its karyotype, its ability to grow in semisolid agar, and in the possession o...
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Synthesis of sulphated proteoglycans by cell lines derived from explants of 7 rheumatoid and 9 normal specimens of synovial tissue, as well as by 7 lines of skin fibroblasts from non-rheumatoid patients, was examined. Cells of all 3 types were cultured as monolayers. They were then disaggregated and their capacity to synthesise proteoglycan estimated in cell suspensions by the incorporation of ...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1967
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.126.6.1005