Cargo- and compartment-selective endocytic scaffold proteins

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Cargo- and compartment-selective endocytic scaffold proteins.

The endocytosis of membrane receptors is a complex and tightly controlled process that is essential for maintaining cellular homoeostasis. The removal of receptors from the cell surface can be constitutive or ligand-induced, and occurs in a clathrin-dependent or -independent manner. The recruitment of receptors into specialized membrane domains, the formation of vesicles and the trafficking of ...

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The endocytic recycling compartment (ERC) is a series of perinuclear tubular and vesicular membranes that regulates recycling to the plasma membrane. Despite evidence that cargo is sorted at the early/sorting endosome (SE), whether cargo mixes downstream at the ERC or remains segregated is an unanswered question. Here we use three-dimensional (3D) structured illumination microscopy and dual-cha...

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M icrotubule-based kinesin motor proteins have many cellular functions, including the transport of various cargoes to different parts of the cell (1). Kanai et al. uncover an unexpected , motor-independent function for one kinesin, KIF13B, in promoting the endocy-tosis of a membrane receptor that regulates cholesterol levels in the blood (2). There are 45 kinesins in the human genome, and the f...

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PAIRing Up Cargo Proteins

All newly made proteins destined to be secreted from our cells or to function at the cell surface—and many of those that function in membranes within the cell— start their lives in a convoluted heap of membranes within cells called the endoplasmic reticulum (ER). From there, they begin a journey that will take them through the stacked membrane compartments of the Golgi apparatus to their eventu...

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Regulation of Endocytic Clathrin Dynamics by Cargo Ubiquitination

VIDEO ABSTRACT Some endocytic cargoes control clathrin-coated pit (CCP) maturation, but it is not known how such regulation is communicated. We found that μ-opioid neuropeptide receptors signal to their enclosing CCPs by ubiquitination. Nonubiquitinated receptors delay CCPs at an intermediate stage of maturation, after clathrin lattice assembly is complete but before membrane scission. Receptor...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 2004

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj20040913