Carboxy-terminal determinants of intracellular protein degradation.

Carboxy-terminal Determinants of Conductance in Inward-rectifier K Channels

Previous studies suggested that the cytoplasmic COOH-terminal portions of inward rectifier K channels could contribute significant resistance barriers to ion flow. To explore this question further, we exchanged portions of the COOH termini of ROMK2 (Kir1.1b) and IRK1 (Kir2.1) and measured the resulting single-channel conductances. Replacing the entire COOH terminus of ROMK2 with that of IRK1 de...

Role of glutathione in intracellular amyloid-alpha precursor protein/carboxy-terminal fragment aggregation and associated cytotoxicity.

Abstract Alterations in glutathione (GSH) metabolism are associated with neurodegeneration in Alzheimer's disease (AD), and GSH depletion follows application of exogenous fibrillar amyloid beta (Abeta) peptides in experimental systems; these results are commonly cited as evidence of oxidative damage in AD. We used MC65 human neuroblastoma cells that conditionally express carboxy-terminal fragme...

Intracellular protein degradation.

The steady-state level of protein in the whole body or in individual tissues is governed not only by the rate of synthesis but by the rate of degradation. Thus protein turnover is a composite term that relates the synthetic and degradative processes. We now have a detailed knowledge of the machinery for synthesizing polypeptide chains and of the requirement for energy to drive the operation, an...

Intracellular Protein Degradation

Between the 1950s and 1980s, scientists were focusing mostly on how the genetic code is transcribed to RNA and translated to proteins, but how proteins are degraded has remained a neglected research area. With the discovery of the lysosome by Christian de Duve it was assumed that cellular proteins are degraded within this organelle. Yet, several independent lines of experimental evidence strong...

Intracellular protein degradation