Capsule of Escherichia coli K29: ultrastructural preservation and immunoelectron microscopy

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Capsule of Escherichia coli K29: ultrastructural preservation and immunoelectron microscopy.

The polysaccharide capsule of Escherichia coli K29 fully surrounds the microorganism and thus occupies an extracellular space ca. 20 times larger in volume than that of the decapsulated cell. Since more than 95% of the capsule consists of water, dehydration for electron microscopy causes the material to collapse. We describe here a method for embedding the capsule in an uncollapsed form. Dehydr...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1985

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.162.3.985-991.1985