CanB, a Druggable Cellular Target in <i>Mycobacterium tuberculosis</i>

Treatment against tuberculosis can lead to the selection of drug-resistant Mycobacterium strains. To tackle this serious threat, new targets from M. are needed develop novel effective drugs. In work, we aimed provide a possible workflow validate and inhibitors by combining genetic, in silico, enzymological approaches. CanB is one three β-carbonic anhydrases that catalyze reversible reaction CO2 hydration form HCO3– H+. end, precisely demonstrated essential for survival pathogen vitro constructing conditional mutants. addition, search inhibitors, canB mutants were also constructed using Pip-ON system. By molecular docking minimum inhibitory concentration assays, selected molecules inhibit growth wild-type strain mutants, thus implementing target-to-drug approach. The compound showed bactericidal activity time-killing assay. We further studied interactions these with enzymatic assays differential scanning fluorimetry thermal shift analysis. conclusion, compounds identified silico screening proved have high affinity as ligands endowed antitubercular activity.